Given below are two statements : Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below :
(1). Statement I is incorrect but statement Il is correct
(2). Both statement I and statement Il are correct
(3). Both statement I and statement II are incorrect
(4). Statement I is correct but statement II is incorrect
Number of Attempts: 2
Correct Attempts: 1
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Average Time: 00:02
In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for B-galactoside production and the recombinant plasmid is inserted in an E. coli strain
(1). It will not be able to confer ampicillin resistance to the host cell. (2). The transformed cells will haye the ability to resist ampicillin as well as produce β- galactoside (3). It will lead to lysis of host cell (4). It will be able to produce a novel protein with dual ability.
Number of Attempts: 2
Correct Attempts: 1
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Average Time: 00:02
ldentify the wrong statement with regard to
Restriction Enzymes.
(1). They cut the strand of DNA at palindromic sites.
(2). They are useful in genetic engineering.
(3). Sticky ends can be joined by using DNA ligases.
(4). Each restriction enzyme functions by inspecting-
the length of a DNA sequence.
Number of Attempts: 2
Correct Attempts: 1
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Match the following columns and select the correctoption.
Polymerase chain reaction (PCR) amplifies
DNA following the equation.
(1). 2N2 (2). N2 (3). 2n (4). 2n+1
Number of Attempts: 2
Correct Attempts: 1
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Which of the following genetically engineered
organisms was used by Eli Lilly to prepare
human insulin?
(1). Phage
(2). Bacterium
(3). Yeast (4). Virus
Number of Attempts: 2
Correct Attempts: 1
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In the above represented plasmid an alien pieceof DNA is inserted af EcoRI site. Which of thefollowing strategies will be chosen to select therecombinant colonies?
(1). Blue color colonies grown on ampicillinplates can beselected. (2). Using ampicillin & tetracyclin containingmedium plate. (3). Blue color colonies will be selected. (4). White color cofonies will be selected.
Number of Attempts: 2
Correct Attempts: 1
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The blue and white selectable markers have been
developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method: Statement I : The blue coloured colonies have
DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements, choose the most appropriate answer from the options given below :
(1). Statement I is incorrect but Statement II
is correct
(2). Both Statement I and Statement II
are correct
(3). Both Statement I and Statement II
are incorrect
(4). Statement I is correct but Statement Il is incorrect
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below :
(1). B and C only
(2). C and D only
(3). A and B only
(4). D and E only
Number of Attempts: 2
Correct Attempts: 1
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Why can't insulin be given orally to diabetic patients?
(1). Its bioavailability will be increased (2). Human body will elicit strong immune response
(3). It will be digested in Gastro-Intestinal (Gl) tract
(4). Because of structural variation
Number of Attempts: 2
Correct Attempts: 1
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Silencing of specific mRNA is possible via
RNAi because of -
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
(1). C (2). A (3). B (4). D
Number of Attempts: 2
Correct Attempts: 1
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
(1). 4 bp (2). 10 bp (3). 8 bp (4). 6 bp
Number of Attempts: 2
Correct Attempts: 1
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below :
(1). B and C only (2). A and E only (3). A and B only (4). D and E only
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:
(1). The gene ' X ' is for protein involved in replication of Plasmid and ' Y ' for resistance to antibiotics . (2). Gene ' X ' is responsible for recognition sites and ' Y ' is responsible for antibiotic resistance . (3). The gene ' X ' is responsible for resistance to antibiotics and ' Y ' for protein involved in the replication of Plasmid . (4). The gene ' X ' is responsible for controlling the copy number of the linked DNA and ' Y ' for protein involved in the replication of Plasmid .
Number of Attempts: 2
Correct Attempts: 1
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The "Ti plasmid" of Agrobacterium tumefaciens stand for
(1). Bio - reactors are used to produce small scale bacterial cultures . (2). Bio - reactors have an agitator system , an oxygen delivery system and foam control system . (3). A bio - reactor provides optimal growth conditions for achieving the desired product . (4). Most commonly used bio - reactors are of stirring type .
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
During the purification process for recombinant
DNA technology, addition of chilled ethanol
precipitates out
(1). DNA
(2). Histones (3). Polysaccharides (4). RNA
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
In gene gun method used to introduce alien DNA
into host cells, microparticles of _______ metal are used.
(1). Zinc
(2). Tungsten or gold
(3). Silver
(4). Copper
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
(1). Bright blue colour
(2). Bright yellow colour
(3). Bright orange colour
(4). Bright red colour
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Main steps in the formation of Recombinant DNA
are given below. Arrange these steps in a correct
sequence.
A. Insertion of recombinant DNA into the host
cell.
B. Cutting of DNA at specific location by
restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given
below:
(1). C, A, B, D
(2). C, B, D, A (3). B, D, A, C (4). B, C, D, A
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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Which of the following is not a cloning vector ?
(1). YAC
(2). pBR322 (3). Probe (4). BAC
Number of Attempts: 2
Correct Attempts: 1
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Given below are two statements: Statement I : Restriction endonucleases recognise specific sequences to cut DNAknown as palindromic nucleotide sequences. Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the
palindromic site.
In the light of the above statements, choose the
most appropriate answer from the options given
below:
(1). Both Statement I and Statement II are correct
(2). Both Statement I and Statement II are incorrect
(3). Statement I is correct but Statement II is
incorrect
(4). Statement I is incorrect but Statement II is correct
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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Which of the following is not a desirable feature of a cloning vector ?
(1). Presence of origin of replication
(2). Presence of a marker gene
(3). Presence of single restriction enzyme site
(4). Presence of two or more recognition sites
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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Given below are two statements: one is labelled
as Assertion (A) and the other is labelled as
Reason (R). Assertion (A): Polymerase chain reaction is
used in DNA amplification. Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the
correct answer from the options given below:
(1). Both (A) and (R) are correct and (R) is the correct explanation of (A)
(2). Both (A) and (R) are correct but (R) is not the correct explanation of (A)
(3). (A) is correct but (R) is not corr
(4). (A) is not correct but (R) is correct
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Which one of the following statements is not true
regarding gel electrophoresis technique?
(1). The process of extraction of separated DNA
strands from gel is called elution
(2). The separated DNA fragments are stained by
using ethidium bromide
(3). The presence of chromogenic substrate gives
blue coloured DNA bands on the gel
(4). Bright orange coloured bands of DNA can be
observed in the gel when exposed to UV light
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Which of the following is a correct sequence of steps
in a PCR (Polymerase Chain Reaction)?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:
(1). Yellow bands (2). Bright orange bands (3). Dark red bands (4). Bright blue bands
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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Which of the following is not an application of PCR (Polymerase Chain Reaction)?
(1). Molecular diagnosis (2). Gene amplification (3). Purification of isolated protein (4). Detection of gene mutation
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :
(1). RNA (2). DNA (3). Histones (4). Polysaccharides
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA ina clone of cells, followed by its detection using autoradiography because:
(1). Mutated gene partially appears on a photographic film. (2). Mutated gene completely and clearly appears on a photographic film. (3). Mutated gene does not appear on a photographic film as the probe has no complementarity with it. (4). Mutated gene does not appear on photographic film as the probe has complementarity with it.
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
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In gel electrophoresis, separated DNA fragments can be visualized with the help of:
(1). Ethidium bromide in UV radiation
(2). Acetocarmine in UV radiation
(3). Ethidium bromide in infrared radiation
(4). Acetocarmine in bright blue light
Number of Attempts: 2
Correct Attempts: 1
Time Taken: 00:04
Average Time: 00:02
The specific palindromic sequence which is
recognized by EcoRI is:
Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.
(1). The enzyme binds DNA at specific sites and cuts only one of the two strands.
(2). The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
(3). The enzyme recognizes a specific palindromic nucleotide sequence in the DNA.
(4). The enzyme cuts DNA molecule at identified position with the DNA.
Number of Attempts: 2
Correct Attempts: 1
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Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
(1). Negatively charged fragments do not move
(2). The larger the fragment size , the farther it moves
(3). The smaller the fragment size , the farther it moves
(4). Positively charged fragments move to farther end
Number of Attempts: 2
Correct Attempts: 1
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A gene whose expression helps to identify transformed cell is known as
