Given below are two statements :
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below :

In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?

Match List - I with List - II.

Choose the correct answer from the options given below.

Plasmid pBR322 has PstI restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for B-galactoside production and the recombinant plasmid is inserted in an E. coli strain

ldentify the wrong statement with regard to Restriction Enzymes.

Match the following columns and select the correctoption.

DNA fragments are

Which of following is wrongly matched in the given table ?

The Taq polymerase enzyme is obtained from

Polymerase chain reaction (PCR) amplifies DNA following the equation.

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

In the above represented plasmid an alien pieceof DNA is inserted af EcoRI site. Which of thefollowing strategies will be chosen to select therecombinant colonies?

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method:
Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.
Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements, choose the most appropriate answer from the options given below :

Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below :

Why can't insulin be given orally to diabetic patients?

Silencing of specific mRNA is possible via RNAi because of -

Identify the part of a bio-reactor which is used as a foam braker from the given figure.

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below :

The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:

The "Ti plasmid" of Agrobacterium tumefaciens stand for

Which of the following statements is incorrect?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

In gene gun method used to introduce alien DNA into host cells, microparticles of _______ metal are used.

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:

Which of the following is not a cloning vector ?

Given below are two statements:
Statement I : Restriction endonucleases recognise specific sequences to cut DNAknown as palindromic nucleotide sequences.
Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site.
In the light of the above statements, choose the most appropriate answer from the options given below:

Which of the following is not a desirable feature of a cloning vector ?

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A): Polymerase chain reaction is used in DNA amplification.
Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements, choose the correct answer from the options given below:

Which one of the following statements is not true regarding gel electrophoresis technique?

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA ina clone of cells, followed by its detection using autoradiography because:

In gel electrophoresis, separated DNA fragments can be visualized with the help of:

The specific palindromic sequence which is recognized by EcoRI is:

Match the organism with its use in biotechnology.

Select the correct option from the following:

Bt cotton variety that was developed by the halten introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with

Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?

The correct order of steps in Polymerase Chain Reaction (PCR) is

The process of separation and purification of expressed protein before marketing is called

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

A gene whose expression helps to identify transformed cell is known as

The DNA fragments separated on an agarose gel can be visualised after staining with

Which of the following is a restriction endonuclease?

Which of the following is not required for any of the techniques of DNA fingerprinting available at present ?

Which of the following is not a feature of the plasmids ?